rabbit polyclonal anti-fto  (Thermo Fisher)

Journal: Virus Research

Article Title: Enterovirus 71 structural viral protein 1 promotes the expression of PMP22 through m 6 A modification in mouse Schwann cells

doi: 10.1016/j.virusres.2025.199590

Figure Lengend Snippet: VP1 up-regulated the expressions of key elements involved in m 6 A modification in MSCs. A-I, qRT-PCR analysis of the mRNA levels of EV71-VP1, METTLE 3/14, YTHDC1, YTHDF 1/2/3, FTO and ALKBH5 in MSCs transfected with PEGFP-C3-VP1 plasmid for 24 h. N = 3. * p < 0.05 compared with the NC group. J-K, Western blot analysis and quantification of protein expressions of EV71-VP1 and key elements involved in m 6 A modification in MSCs transfected with PEGFP-C3-VP1 plasmid for 48 h, including ALKBH5, FTO, METTLE 3/14, YTHDF 1/2/3, and YTHDC1. GAPDH was used as internal control. N = 3. * p < 0.05 compared with the NC group.

Article Snippet: The primary antibodies used in this study were: anti-GAPDH antibody (1:1000, Abcam, Cat. # ab181602); Enterovirus 71 VP1 monoclonal antibody (1:1000, Thermo Fisher, Cat. # MA5–33,257); Rabbit anti-METTL14 antibody (1:1000, Bioss, Cat. # bs-17608R); Rabbit anti-FTO antibody (1:1000, Bioss, Cat. # bs-7056R); Rabbit anti-ALKBH5 antibody (1:1000, Bioss, Cat. # bs-20540R); Recombinant anti-METTL3 antibody [ EPR18810 ] (1:1000, Abcam, Cat. # ab195352); Recombinant Anti-YTHDF1 antibody [ EPR22349 –16] (1:1000, Abcam, Cat. # ab252346); Recombinant Anti-YTHDF2 antibody [ EPR20318 ] (1:1000, Abcam, Cat. # ab220163); Recombinant Anti-YTHDF3 antibody [ EPR21912 –3] (1:1000, Abcam, Cat. # ab220161); Recombinant Anti-YTHDC1 antibody [ EPR21821 ] (1:1000, Abcam, Cat. # ab220159).

Techniques: Modification, Quantitative RT-PCR, Transfection, Plasmid Preparation, Western Blot, Control